Nanomaterials based on carbon framework for cells stimulation

The work is devoted to the technology of formation and study of physical and chemical characteristics and biocompatibility of nanomaterials based on hybrid structures of carbon nanotubes and reduced graphene oxide, obtained by spray deposition and laser processing with nanosecond pulsed laser of 1064 nm wavelength, for bioelectronics applications. The formation of a connected structure between the components was proved with scanning electron microscopy, the optimal power of laser processing was found as 0.07 W to obtain the largest number of connections between carbon nanotubes and reduced graphene oxide, thanks to which high electrical conductivity was achieved. The experiments on electrical stimulation in vitro were provided with the developed setup based on a culture plate, an impulse generator and electrodes connected to samples. Cells seeded on the obtained structures with electrical stimulation demonstrate better proliferation and monolayer forming compared to the control sample. Thus, the developed structures can be successfully used as part of various bioelectronic devices for improved tissue recovery using electrical stimulation.