Photo-assisted adsorption of enzyme molecules onto a surface-modified silicon substrate

In this work, the influence of illumination on the adsorption of enzyme molecules from an aqueous solution on a single-crystal silicon substrate with a layer of amorphous silicon (a-Si) is shown by atomic force microscopy. It was shown that the effect of illumination during the formation of an enzyme layer depends both on the type of Si conductivity and on the presence of an a-Si layer on the surface. The 2-beam interference pattern on the surface of the n-Si/a-Si structure, fabricated by illumination with a wavelength of 491 nm before the adsorption process, made it possible to fabricate ordered rows of the precipitated enzyme. This pattern not observed for p-Si/a-Si structure or bare substrate of single-crystal Si without the amorphous silicon layer. The developed technique is promising for the fabrication of multienzyme coatings for multiplex analysis using silicon transducer.