Tissue reconstruction of skin failues and soft-tissue injuries using regenerative medicine methods

Biophysics and medical physics

The electrospinning technique has been used to manufacture the composite material based on nanofibers made of aliphatic copolyamide and composite nanofibers made of chitosan and chitin nanofibrils. Experimental in vivo studies of the material worked out as wound covering were carried out to treat the vast and multilayered wound on the rat’s back. After 28 days and nights of observation the complete epithelialization of the wound surface was established to occur in the experimental rat group. Histological analysis of scar tissue showed the presence of small minority of capillaries and a low amount of infiltrate cells. The survival of animals was 100 %. At the same time, in the control group of animals, the lethality was observed in 11 % of cases, and suppurative complications were observed in 100 % of cases. To increase the rate of tissue regeneration, and to reduce the infection probability the thrombocyte gel prepared from the peripheral blood of the patients was used. The curative effect was proved to increase when applied simultaneously the wound coatings and autologous blood product separation – thrombocyte gel and platelet-rich plasma.