Structural stability of the villin domain 6 in the absence of calcium ions

Biophysics and medical physics

Using methods of solution heteronuclear NMR spectroscopy we investigate the structural stability of domain 6 of actin-binding protein Villin (V6) in the absence of calcium ions. We have demonstrated calcium-free V6 to have a stable tertiary structure that was markedly different from the previously reported solution structure of the calcium-bound domain.