The estimation of hard-to-measure physical parameters of thermal dissociation of biomolecules from results of indirect measurements

Simulation of physical processes

The present paper is devoted to finding the necessary minimum of experimental information on biomolecules for quantitative evaluation of such physical parameters which cannot be directly measured for some reason, but are connected by known mathematical relations to any measureable quantities. For the case when thermal dissociation of a complex molecule is possible through several channels due to breaking of various intramolecular bonds, an original analytic expression relating the association degree of biomolecules to its physical parameters and the environment temperature has been deduced. It was exemplified the possibility to evaluate (with satisfactory accuracy) some physical parameters of thermal dissociation protease SARS-CoV-2 dimer and the temperature dependence of the association degree of this dimer as well.