The fluorescent microscope upgrading to investigate biological structures with subdiffractional resolution

Experimental technique and devices

An upright fluorescence microscope, which is a part of the Optical Tweezers set-up, is upgraded to perform localization microscopy. The resolution of 50 nm was obtained with a test object. Recommendations on upgrading already existing or newly bought microscopes to perform localization microscopy and on calibrating and testing upgraded microscopesare proposed.