Tightly-focused femtosecond laser radiation is widely used as a convenient tool for manipulating biological objects. The method of femtosecond laser surgery proved to be useful for the multiple applications, from eye correction to the subcellular structures destruction. Among other things, femtosecond laser surgery shown to be used as a tool for DNA destruction, which could be beneficial for assisted reproductive technologies in humans. In the context of the development of this method, the study of the DNA laser assisted destruction efficiency is of significant importance. In this work, DNA laser assisted destruction efficiency in presence of SYTO fluorescent dye was studied.