St. Petersburg Polytechnic University Journal: Physics and Mathematics Научно-технические ведомости СПбГПУ. Физико-математические науки 2304-9782, 2618-8686, 2405-7223 18 Changing of DNA molecule mechanical properties during interaction with YOYO-1 intercalating dye Изменение механических свойств молекулы ДНК при взаимодействии с интеркалирующим красителем YOYO-1 0000-0003-0836-0732 Pobegalov Georgiy lwdrums@gmail.com 0000-0003-0901-4188 Arseniev Anatoly arsenievanatoly@gmail.com Vedyaykin Alexey Sokolova Maria Fedorova Yana Sabantsev Anton Peter the Great St. Petersburg Polytechnic University 10 03 2013 1 165 136 143

The method for registration of a single DNA molecule length change during interaction with a ligand is proposed. The method is tested in the case of DNA molecule interaction with intercalating YOYO-1 dye. The experimental data obtained signifies both DNA elongation and mechanical properties alteration upon YOYO-1 binding.

The proposed method can be used to study single DNA molecule interaction with different ligands, including dyes and DNA-binding proteins. Also this method can be used to study other biopolymers, including amyloid fibrils.

 fluorescence microscopy optical trapping laser tweezers set-up DNA YOYO-1 intercalation