Processing of qPCR signals obtained on microfluidic chips in the measurement sequence disorder event


The formation and movement of gas bubbles in microfluidic chips leads to the measurement sequence disorder in the form of a signal distortion. Two new methods for automatic processing of distorted qPCR signals were developed and implemented. Methods are based on the qPCR signals approximation by a sigmoid function and make it possible to successfully perform a microfluidic chips qPCR analysis in the event of bubbles, which is demonstrated on experimental and simulation curves.