Bisretinoid N-retinyl-N-retinylidene ethanolamine (A2E) of the retinal pigment epithelium (RPE) lipofuscin granules is a side product of visual cycle. Its accumulation is associated with degenerative diseases of the retina and retinal pigment epithelium. In this study A2E photooxidation and photodegradation products were studied. Absorption and 2D fluorescence spectra of these substances were detected. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) and Fourier-transform infrared spectroscopy (FTIR) revealed chemical changes during the A2E photooxidation process. Aldehyde accumulation was observed and new structure one of the resulting compounds was proposed.