Coatings of titanium dioxide nanotubes were obtained on glass substrates by hydrothermal synthesis method. The influence of the seed layer synthesis method on the coatings quality was studied. The synthesized materials are nanoscale, crystallized in a mixed anatase-rutile modification. It was shown that for seed layers deposited by low-temperature solid-phase pyrolysis technique, the coatings are more homogeneous than for seed layers deposited by sol-gel method. In addition, a tendency to agglomeration was noted for nanotubes grown on a seed layer deposited by the sol-gel method. The electrophysical properties study showed that for materials with a seed layer obtained by the sol-gel method, the resistance is an order of magnitude higher than for materials with a seed layer synthesized by low-temperature pyrolysis technique. The activation energy (Ea) for titanium dioxide nanotubes (seed layer is applied by sol-gel method) was 0.74 eV, and for the sample obtained by low-temperature pyrolysis Ea was 0.68 eV.