Applying femtosecond lasers in assisted reproductive technologies is very promising. The removing of a pronucleus (called epronucleation) in three-pronuclear zygotes may help to increase the number of normal diploid embryos. In this work we demonstrated the possibility of epronucleation in mouse zygotes by the femtosecond laser irradiation. We showed that the femtosecond laser could effectively destroy the pronuclei. At the same time, zygotes retained their viability and did not destroy.