The nature of intermolecular interactions between glucose and a molecularly imprinted polymeric sorbent — gl-MlP has been investigated. The sorption isotherms and thermodynamic functions testified to energetically heterogenous distribution of glucose in the sorbent phase. The contribution of specific binding to the equilibrium factors of the distribution and the change of the glucose sorption free energy increased in the physico-chemical conditions the closest to the gl-MlP synthesis conditions.